Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma genitalium without cross-reaction with other biological genomes.

2. High sensitivity, 100% detection of 10 genomes per reaction.

3. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.

 
Mycoplasma genitalium is a sexually transmitted pathogenic bacterium that is obligately intracellular and lives on ciliated epithelial cells of the human urethra and reproductive tract. It can also be found in respiratory, rectal and synovial fluid samples. exist. Its complete genome sequence was published in 1995, with a size of 0.58 Mb and only 482 protein-coding genes. It is the smallest self-replicating prokaryotic cell and the second completely sequenced bacterial genome after Haemophilus influenzae. Mycoplasma genitalium was first isolated from the human urogenital tract in 1981 and was identified as a new mycoplasma species in 1983. It causes urethritis in both men and women, occurs at a higher rate than other common sexually transmitted infections, and is a recognized risk factor for HIV transmission, with higher rates among gay men and men who have previously received the antibiotic azithromycin. According to a synthesis of literature reports, among men, Mycoplasma genitalium is present in 21% of patients with non-gonococcal urethritis, and 6% of asymptomatic patients are carriers; among women, Mycoplasma genitalium is associated with cervicitis, uterine urethritis, and cervicitis. Endonitis, pelvic inflammatory disease and fallopian tube infertility are related, and they are more common in the population.About 2-7.3% are carriers.

Mycoplasma genitalium has extremely high requirements on the culture medium and grows slowly, making it difficult to culture. In particular, culture from clinical specimens is less likely to be successful, which makes detection of pathogens in clinical specimens and subsequent isolation and culture very difficult. Mycoplasma genitalium and Mycoplasma pneumoniae share common antigens, resulting in cross-reactivity in most serological experiments. Since there are no specific serological tests, nucleic acid amplification testing is the only viable option for detecting M. genitalium. The PCR method has the advantages of high sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours. Compared with conventional PCR method, quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

This kit selects a dehydrogenase gene as a target to specifically identify Mycoplasma genitalium, verified by BLAST, has no cross-reactivity with other biological genomes. This kit detected 13 other mycoplasma species and found no non-specific signals; 1,600 cervical swab DNAs were tested and 119 were found to be positive, which was consistent with the test results of the imported S brand qPCR kit and was more sensitive.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测生殖支原体，与其他生物基因组没有交叉反应。

2， 灵敏度高，可百分百检出每反应10个基因组。

3， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

生殖支原体（Mycoplasma Genitalium）是一种性传播的致病菌，专性细胞内寄生，生活在人类尿道和生殖道的纤毛上皮细胞上，在呼吸道、直肠和滑液样本中也可发现其存在。其完整的基因组序列发表于1995年，大小为0.58 Mb，只有482个蛋白编码基因，是最小的可自我复制的原核细胞，也是继流感嗜血杆菌之后第二个完整测序的细菌基因组。1981年首先从人类的泌尿生殖道中分离出生殖支原体，于1983年被确定为新的支原体物种。它在男性和女性中都会导致尿道炎，发病率高于其他常见的性传播感染，也是艾滋病毒传播的一个公认的危险因素，同性恋男性和之前接受过阿奇霉素抗生素治疗的男性患病率较高。根据一份由文献报道综合的数据，在男性中，生殖支原体存在于21%的非淋球菌尿道炎患者，无症状者中有6%为携带者；在女性中，生殖支原体与宫颈炎、子宫内膜炎、盆腔炎症和输卵管不孕有关，在人群中大约2-7.3%为携带者。

生殖支原体对培养基要求极高且生长缓慢,培养较难,尤其是从临床标本中培养更不容易成功，这使得检测临床标本中的病原体和随后的分离培养都非常困难。生殖支原体与肺炎支原体（Mycoplasma Pneumoniae）有共同的抗原，在大多数的血清学实验中形成交叉反应。由于没有特异性的血清学检测方法，核酸扩增试验是检测生殖支原体唯一可行的选择。PCR法具有灵敏度高和特异性强的优点，且检测时间短，仅需几个小时即可获得结果。定量PCR法与常规PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒选择一个脱氢酶基因作为靶点，特异性识别生殖支原体，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了其他13种支原体，未发现非特异信号；对1600个宫颈拭子DNA进行检测，发现119个阳性，与进口S品牌qPCR试剂盒检测结果一致，且灵敏度更高。

 

 

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