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Sunlong Medical™ Mouse TNF-α ELISA Kit
Sunlong Medical™ Mouse TNF-α ELISA Kit
TNF elisa kit | tumor necrosis factor elisa kit | DIF | TNF alpha | TNF-alpha | Tnfa | TNFalpha | Tnfsf1a | tumor necrosis factor-alpha | tumor necrosis factor | alpha
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Regular members: $455.2
  • Save more [Favourable] 45% Discount
  • NO.:EL0004Mo
    Species:Mouse
    Assay range:10.938pg/mL-700pg/mL
    Sensitivity:0.99 pg/mL
    Sample Volume:20 μL
    Validity:Two Years
    Assay type:Sandwich Method
  • Goods click count:335
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Cat NO.:
EL0004Mo
Product:
Sunlong Medical™ Mouse TNF-α ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
81% - 119%
Mean Spike Recovery:
0.99
CV of Intra plate:
4.0 % - 6.1 %
CV of Inter plate:
5.5 % - 7.6 %
NCBI_Gene:
21926
UniProtKB:
P06804

 

PLATFORM ELISA
PLATE Detachable 96-well plate
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse TNF-α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TNF-α present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TNF-α in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Partial purchase records(bought amounts latest6)

UsernameQuantitybought time
Pe***12024-09-07
Em***12024-08-18
Di***12024-07-31
Sa***22024-07-02
Na***32024-02-19
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