Cat NO.:	EL0034Ra
Product:	Sunlong Medical™ Rat IL-6 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	92% - 104%
Mean Spike Recovery:	0.97
CV of Intra plate:	4.2 % - 7.7 %
CV of Inter plate:	6.3 % - 9.1 %
NCBI_Gene:	24498
UniProtKB:	P20607

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Rat IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

1. Identification of Clec7a as the therapeutic target of rTMS in alleviating Parkinson’s disease: targeting neuroinflammation

 

2. HDAC6 inhibition alleviates acute pulmonary embolism: a possible future therapeutic option

 

3. 6-Gingerol attenuates sepsis-induced acute lung injury by suppressing NLRP3 inflammasome through Nrf2 activation

 

4. M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats

 

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7. Gastrodin Prevents Neuronal Apoptosis and Improves Neurological Deficits in Traumatic Brain Injury Rats through PKA/CREB/Bcl2 Axis

 

8. Puerarin Attenuates High-Glucose and High-Lipid-Induced Inflammatory Injury in H9c2 Cardiomyocytes via CAV3 Protein Upregulation

 

9. Astaxanthin prevents osteoarthritis by blocking Rspo2-mediated Wnt/β-catenin signaling in chondrocytes and abolishing Rspo2-related inflammatory factors in macrophages

 

10. SIRT3 confers protection against acute pulmonary embolism by anti-inflammation, anti-oxidative stress, anti-apoptosis: participation of AMPK/mTOR pathway

 

11. SOX9 modulates Müller cell gliosis in diabetic retinopathy through upregulating TXNIP transcription

 

12. Knockdown of HDAC9 attenuates sepsis-induced myocardial injury and inflammatory response

 

13. Lycopene ameliorates locomotor activity and urinary frequency induced by pelvic venous congestion in rats

 

14. Berberin sustained-release nanoparticles were enriched in infarcted rat myocardium and resolved inflammation

 

15. Protective Effects of Aqueous Extracts of the Herb of Paederia scandens (Lour.) Merr. against HCl/EtOH-Induced Gastric Ulcer in Rats: Involvement and Inhibitors’ Identification of NF-κB Signaling

 

16. Baicalin Ameliorates Lung Injury in Rats by Inhibiting NLRP3 Inflammasome Activation via NF-κB Signaling Pathway

 

 

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