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Cat NO.:EL0139Hu Product:Sunlong Medical™ Human IFN-β ELISA Kit Storage4℃ (unopened) for two years Shipping Condition4℃ Sample Type:Serum | plasma | cell culture supernatant and other biological samples Spike Recovery Range:79% - 95% Mean Spike Recovery:0.88 CV of Intra plate:4.5% - 5.0% CV of Inter plate:3.6% - 7.9% NCBI_Gene:3456 UniProtKB:P01574
PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IFN-β monoclonal antibody
Human IFN-β freeze-dried standard
IFN-β detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing FilmASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IFN-β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IFN-β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-β in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
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