SAMPLE TYPE	Serum, plasma, cell culture supernatant, and other biological samples
ASSAY TIME	3.5 h
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	Pre-coated ELISA plate detect Antibody Positive control 10×Assay Buffer Substrate TMB Stop Solution 20×Washing Buffer Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific monoclonal antibodies against mouse immunoglobulin subtypes are precoated on a high-affinity ELISA plate. The ELISA plate wells are added with test samples, positive controls, and HRP-conjugated detection antibodies. After incubation, the immunoglobulins present in the samples bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, TMB chromogenic substrate is added for color development while avoiding light. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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Totalgoods,subtotals:￥Checkout