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Drug Names
Generic Name: Rat Anti-Liver-Kidney Microsomal Antibody 1 (LKM-1) ELISA Kit
Purpose
Our Rat Anti-Liver-Kidney Microsomal Antibody 1 (LKM-1) ELISA kit is to assay LKM-1 levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to LKM-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for LKM-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain LKM-1 and HRP conjugated LKM-1 antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of LKM-1. You can calculate the concentration of LKM-1 in the samples by comparing the OD of the samples to the standard curve.
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