Product Name	DGKB
Chinese Name	甘油二酯激酶β/DGK-β抗体
Alias	90 kDa diacylglycerol kinase; DAG kinase beta; DAGK2; DGK; DGK B; DGK beta; DGK-beta; Dgkb; DGKB_HUMAN; Diacylglycerol kinase beta (90kD); Diacylglycerol kinase beta 90kDa; Diacylglycerol kinase beta; Diglyceride kinase beta; KIAA0718.
Research Area	Tumour Cell biology Signal transduction The new supersedes the old Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse(predicted:Human,Rat,Dog,Cow,Horse,Sheep)
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	91kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DGKB: 1-100/804
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] Function: Exhibits high phosphorylation activity for long-chain diacylglycerols. Subcellular Location: Cytoplasm. Similarity: Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers. SWISS: Q9Y6T7 Gene ID: 1607 Database links: Entrez Gene: 1607 Human Entrez Gene: 217480 Mouse Omim: 604070 Human SwissProt: Q9Y6T7 Human SwissProt: Q6NS52 Mouse Unigene: 567255 Human Unigene: 126525 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGKB) Polyclonal Antibody, Unconjugated (SL14295R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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