Product Name	PAFR/PAF Receptor
Chinese Name	血小板活化因子受体抗体
Alias	Platelet-activating factor receptor; PAF receptor; Ptafr; OTTHUMP00000004041; OTTMUSP00000010350; PAF R; PAF-R; PAFR; Platelet activating factor receptor; Platelet-activating factor receptor; PTAFR; PTAFR_HUMAN; RP23-470B20.1.
literatures	Specific References (3) | SL1478R has been referenced in 3 publications. [IF=4.61] Hammond, Jennetta W., Shao-Ming Lu, and Harris A. Gelbard. "Platelet Activating Factor Enhances Synaptic Vesicle Exocytosis Via PKC, Elevated Intracellular Calcium, and Modulation of Synapsin 1 Dynamics and Phosphorylation." Frontiers in cellular neuroscience 9 (2015). IF(ICC) ; Rat. PubMed:26778968 [IF=4.582] Silvestro Ennio D'Anna. et al. Bacterial load and related innate immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease. RESP MED. 2023 Aug;215:107297 IHC ; Human. PubMed:37245650 [IF=4.174] Jiaqi Jin. et al. Myocardial ischemia-reperfusion injury is probably due to the excessive production of mitochondrial ROS caused by the activation of 5-HT degradation system mediated by PAF receptor. MOL IMMUNOL. 2023 Mar;155:27 WB ; Rat. PubMed:36682136
Research Area	Cardiovascular Cell biology immunology Neurobiology Signal transduction The cell membraneReceptor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Rabbit, )
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1µg/Test （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PAFR: 231-342/342
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The PAF receptor binds platelet-activating factor (PAF) and is thought to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. PAF is a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. It has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes. The PAF receptor is induced by granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-5 and n-butyrate. A diverse range of compounds act as PAF receptor antagonists; these may be useful pharmacologically. Function: Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. Subunit: Interacts with ARRB1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in the placenta, lung, left and right heart ventricles, heart atrium, leukocytes and differentiated HL-60 granulocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25105 Gene ID: 5724 Database links: Entrez Gene: 5724 Human Entrez Gene: 19204 Mouse Entrez Gene: 58949 Rat Omim: 173393 Human SwissProt: P25105 Human SwissProt: Q62035 Mouse SwissProt: Q9XSD4 Pig SwissProt: P46002 Rat SwissProt: P35366 Rhesus monkey Unigene: 433540 Human Unigene: 725944 Human Unigene: 89389 Mouse Unigene: 10137 Rat
Product Picture	Figure 1. Sample: Lane1: Lung(Rat) Lysate at 30 ug Lane2: Brain(Rat) Lysate at 30 ug Primary: Anti-PTAFR (SL1478R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size: 38kD Observed band size: 38kD Figure 2. Sample: Lymph (Mouse) Lysate at 30 ug Primary: Anti- RAFR/PAF (SL1478R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 38 kD Observed band size: 38 kD Figure 3. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated(SL1478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Figure 4. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated(SL1478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Figure 5. Blank control: 293T cells(blue). Primary Antibody:Rabbit Anti-PAFR/PAF antibody(SL1478R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL1478R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed