Drug Names

Generic Name: Human Influenza viruses B (FLU B)ELISA Kit

Purpose

Our Human Influenza viruses BP (FLU B)ELISA Kit is to for the qualitative determination of FLU B in Human serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an Antibody specific to FLU B, make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for FLU B is added to each Microplate well and incubated, so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain FLU B and HRP conjugated FLU B antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of FLU B is determined by comparing with the CUTOFF value.

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