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Standard ：calibration substance with preservatives
Standard diluent 1.5ml：0.01M PBS
HRP-Conjugate reagent 6ml：HRP-linked Avidin
Sample diluent 6ml：1% BSA（CAS # 9048-46-8）, 0.05%Tween-20 PBS
Chromogen Solution A 6ml：32μL30% H2O2(CAS# 7722-84-1)
Chromogen Solution B 6ml：0.5 ml 2 mg/mL TMB（CAS # 54827-17-7）
Stop Solution 6ml ：2mol/L H2SO4(CAS# 7664-93-9)
wash solution 20ml 30X ： 30X TBS
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