Questions for below product:
AK0389-100T-48S Neutral Proteinase(NP) Activity Assay Kit

AK0521-100T-96S Glucose Oxidase(GOD) Activity Assay Kit

AK0210-100T-48S Cellulase(CL) Activity Assay Kit

I noticed in the protocol that there is an extraction step. However, wewill use pure powdered enzyme products to evaluate the enzyme activity.Do you think this extraction step would be necessary?Do you recommend these kits for quality control and shelf testing ofpowdered enzyme products?


English Answer:The function of the extraction solution is to provide the pH of the reaction system. For enzyme products, they can be directly dissolved in the extraction solution and then detected. It is necessary to ensure complete dissolution before detection. In addition, there may be batch - to - batch differences in the reagent kits due to the replacement of raw materials. Generally, this difference is within 10%. Check the requirements of the customer's quality control. If the customer can accept the batch - to - batch differences, it can be used for product quality control. Also, the definitions of enzyme activity in different reagent kits or with different detection principles are different. The customer needs to determine whether the principle of their own detection standard and the definition of enzyme activity are consistent with those of our reagent
kit. If they are consistent, it can be used.

Chinese Answer:提取液的作用是提供反应体系PH的，酶制品的话可以直接提取液溶解后检测，需要完全溶解后再检测的哈。  另外，试剂盒批次间因为原料的更换会有批间差异，一般是10%以内，看下客户质量控制的要求吧 能接受批间差异的话可以用于产品质量控制。还有一点  不同试剂盒或者不同检测原理酶活性定义不一样，需要客户判断下自己检测标准的原理以及酶活性定义是否和咱们的试剂盒一致  一致的话可以用