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Product Name Ubiquityl-Histone H2B (Lys120) Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. A12B4 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:1000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG2a/Kappa Purification Antigen affinity purification Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 核小体将DNA包裹并压缩成染色质,限制了DNA进入需要DNA作为模板的细胞机制。因此,组蛋白在转录调控,DNA修复,DNA复制和染色体稳定性等方面起着重要作用。组蛋白H2B第120位赖氨酸单Ubiquitin化是一种重要的组蛋白修饰,在许多物种的基因转录调控和高级染色质组织中起着重要作用。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, HepG2, Neuro-2a
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 23 kDa
Observed MW: 23 kDa
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60166M
Tissue: Rat liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60166M
Tissue: Mouse kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60166M
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