Product Name	Malonyl-Histone H3 (Lys122)
Chinese Name
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	H2D1
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-1:2000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Antigen affinity purification
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	赖氨酸丙二酰化是一种最近发现的可逆修饰，可调控蛋白质活性。Sirt5是一种赖氨酸丙二酸化修饰调节酶，可催化体内外赖氨酸脱丙二酰化。 整合蛋白质组学和生物化学分析技术发现，原核生物和真核生物中，不管是组蛋白还是非组蛋白都可发生赖氨酸丙二酰化。赖氨酸丙二酰化可能为研究胞内调节如各种生物体中的能量代谢提供新的典范。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney 6: Recombinant histone H3(20ng) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 15 kDa Observed MW: 15 kDa Tissue: Mouse liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60210R Tissue: Rat liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60210R Tissue: Human breast Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60210R

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