BCA Protein Assay Kit

Cat: AK0651-50T

Size: 50T

Storage: BSA protein standard keeps at 4℃ for 3 months，or at -20℃ for 1 year, other reagents keep at room temperature for 1 year.

Components:

	50T		Storage
BCA reagent	100ml		RT
Cu reagent	3ml		RT
PBS dilution	30ml		RT
BSA Standard(5mg/ml)	1ml		-20℃

Product Description:

The BCA Protein Assay Kit is based on the protein reduces Cu2+ to Cu+ and the principle that BCA forms a purple-blue complex with Cu1+ under alkaline condition, followed by detecting absorbance at 562nm and comparing with standard curve to quantify the protein. Commonly used detergents SDS, Triton X-100, Tween do not affect the test results, but The BCA Protein Assay Kit is affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. During the experiments, when the high background caused by sample dilution or lysate, it is advisable to take  the Bradford Protein Assay Kit into account.