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Human P-selectin glycoprotein  ligand 1 (PSGL-1) ELISA Kit

Human P-selectin glycoprotein  ligand 1 (PSGL-1) ELISA Kit

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  • Cat NO.:SL3736Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:15pg/ml-500pg/ml
  • Sensitivity:1pg/ml
  • Standard:540pg/ml
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic Name: Human P-selectin glycoprotein ligand 1 (PSGL-1) ELISA Kit

Purpose

Our Human P-selectin glycoprotein ligand 1 (PSGL-1) ELISA kit is to assay PSGL-1 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PSGL-1, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for PSGL-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PSGL-1 and HRP conjugated PSGL-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PSGL-1. You can calculate the concentration of PSGL-1 in the samples by comparing the OD of the samples to the standard curve.

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