Storage: 2-8 ℃.  Period of validity: One year.  Date of production: see the package

Principle:

The enzyme horseradish peroxidase (HRP, Molecular weight: 40000) forms a colored (brown), insoluble precipitate at the antigenic sites in
the presence of a substrate (H2O2) using a chromogen, DAB (3, 3'-diaminobenzidine) that is easily visualized with light microscopy. The colored
precipitate from DAB is stable for several years providing a permanent record.

50T Reagents supplied：

		Size
1	Citrate buffer (0.01mol/L, pH6.0)(Powder)	Soluble in 0.5L deionized water or distilled water
2	PBS buffer(Powder)	Soluble in 1L deionized water or distilled water
3	DON-HRP-Conjugated polyase  (anti mouse / rabbit)	1.5mL
4	30% H2O2	5mL
5	DAB solution I	0.3mL
6	DAB solution II	0.3mL

100T Reagents supplied：

		Size
1	Citrate buffer (0.01mol/L, pH6.0)(Powder)	Soluble in 1L deionized water or distilled water
2	PBS buffer(Powder)	Soluble in 2L deionized water or distilled water
3	Enzyme labeled secondary antibody polymer (anti mouse / rabbit)	3mL
4	30% H2O2	10mL
5	DAB solution I	0.6mL
6	DAB solution II	0.6mL

200T Reagents supplied：

		Size
1	Citrate buffer (0.01mol/L, pH6.0)(Powder)	Soluble in 2L deionized water or distilled water
2	PBS buffer(Powder)	Soluble in 4L deionized water or distilled water
3	Enzyme labeled secondary antibody polymer (anti mouse / rabbit)	6mL
4	30% H2O2	20mL
5	DAB solution I	1.2mL
6	DAB solution II	1.2mL

Materials required but not supplied: ethanol, hematoxylin