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IHC kit, Immunohistochemistry kit

  • Cat NO.:SL-IHC
  • Product Spec:50T
  • 100T:280
  • 200T:500
  • Price:$150
  • Total:
  • Quantity:
  • Manual

Goods Brief:

 Storage: 2-8 .  Period of validity: One year.  Date of production: see the package

Principle:

The enzyme horseradish peroxidase (HRP, Molecular weight: 40000) forms a colored (brown), insoluble precipitate at the antigenic sites in
the presence of a substrate (H2O2) using a chromogen, DAB (3, 3'-diaminobenzidine) that is easily visualized with light microscopy. The colored
precipitate from DAB is stable for several years providing a permanent record.

50T Reagents supplied

 

 

Size

1

Citrate buffer (0.01mol/L, pH6.0)(Powder)

Soluble in 0.5L deionized water or distilled water

2

PBS buffer(Powder)

Soluble in 1L deionized water or distilled water

3

DON-HRP-Conjugated polyase

 (anti mouse / rabbit)

1.5mL

4

30% H2O2

5mL

5

DAB solution I

0.3mL

6

DAB solution II

0.3mL

100T Reagents supplied

 

 

Size

1

Citrate buffer (0.01mol/L, pH6.0)(Powder)

Soluble in 1L deionized water or distilled water

2

PBS buffer(Powder)

Soluble in 2L deionized water or distilled water

3

Enzyme labeled secondary antibody polymer (anti mouse / rabbit)

3mL

4

30% H2O2

10mL

5

DAB solution I

0.6mL

6

DAB solution II

0.6mL

200T Reagents supplied

 

 

Size

1

Citrate buffer (0.01mol/L, pH6.0)(Powder)

Soluble in 2L deionized water or distilled water

2

PBS buffer(Powder)

Soluble in 4L deionized water or distilled water

3

Enzyme labeled secondary antibody polymer (anti mouse / rabbit)

6mL

4

30% H2O2

20mL

5

DAB solution I

1.2mL

6

DAB solution II

1.2mL


Materials required but not supplied: ethanol, hematoxylin 

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