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Human Insulin like Growth Factor  Binding Protein 7 (IGFBP-7)  ELISA Kit

Human Insulin like Growth Factor  Binding Protein 7 (IGFBP-7) ELISA Kit

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  • Cat NO.:SL3534Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:1.3 μg/L - 40μg/L
  • Sensitivity:0.1 μg/L
  • Standard:45 μg/L
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameHuman Insulin like Growth Factor Binding Protein 7 (IGFBP-7) ELISA Kit

Purpose

Our Human Insulin like Growth Factor Binding Protein 7 (IGFBP-7) ELISA kit is to assay IGFBP-7 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IGFBP-7. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IGFBP-7 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IGFBP-7 and HRP conjugated IGFBP-7 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IGFBP-7. You can calculate the concentration of IGFBP-7 in the samples by comparing the OD of the samples to the standard curve.

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