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Human anti aqua-4porin (AQp4-Ab)ELISA Kit

Human anti aqua-4porin (AQp4-Ab)ELISA Kit

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  • Cat NO.:SL3351Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:12ng/L -400ng/L
  • Sensitivity:1ng/L
  • Standard:450ng/L
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameHuman anti aqua-4porin (AQp4-Ab)ELISA Kit

Purpose

Our Human anti aqua-4porin (AQp4-Ab)ELISA Kit is to assay AQp4-Ab levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to AQp4-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for AQp4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AQp4-Ab and HRP conjugated AQp4 antigen will appear blue in color and then turn yellow after the addition of  the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AQp4-Ab. You can calculate the concentration of AQp4-Ab in the samples by comparing the OD of the samples to the standard curve.

 

 

 

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