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Rat Neurofilament, Light Polypeptide (NEFL)  ELISA Kit

Rat Neurofilament, Light Polypeptide (NEFL) ELISA Kit

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  • Cat NO.:SL1564Ra
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:3 pg/ml -200 pg/ml
  • Sensitivity:0.5 pg/ml
  • Standard:270 pg/ml
  • Price:$498
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameRat Neurofilament, Light Polypeptide (NEFL)ELISA Kit

Purpose

Our Rat Neurofilament, Light Polypeptide (NEFL) ELISA kit is to assay NEFL levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NEFL. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NEFL is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NEFL and HRP conjugated NEFL antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NEFL. You can calculate the concentration of NEFL in the samples by comparing the OD of the samples to the standard curve.

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