Drug Names

Generic Name：Rat Glucose-6-phosphatase (G-6-Pase) ELISA Kit

Purpose

Our Rat Glucose-6-phosphatase(G-6-Pase) ELISA kit is to assay G-6-Pase levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to G-6-Pase. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for G-6-Pase is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain G-6-Pase and HRP conjugated G-6-Pase antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of G-6-Pase. You can calculate the concentration of G-6-Pase in the samples by comparing the OD of the samples to the standard curve.