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Rat Granulocyte-Macrophage Colony Stimulating Factor(PC)ELISA Kit

Rat Granulocyte-Macrophage Colony Stimulating Factor(PC)ELISA Kit

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  • Cat NO.:SL1562Ra
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:15 ng/L-3000 ng/L
  • Sensitivity:7.36 ng/L
  • Standard:3600 ng/L
  • Price:$498
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameRat Granulocyte-Macrophage Colony Stimulating Factor (PC) ELISAKit

Purpose

Our Rat Granulocyte-Macrophage Colony Stimulating Factor(PC) ELISA kit is to assay PC levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PC. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for PC is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PC and HRP conjugated PC antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PC. You can calculate the concentration of PC in the samples by comparing the OD of the samples to the standard curve.

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