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Rat Prothrombin Fragment 1+2 (F1+2) ELISA Kit

Rat Prothrombin Fragment 1+2 (F1+2) ELISA Kit

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  • Cat NO.:SL1539Ra
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:0.6pg/ml -40 pg/ml
  • Sensitivity:0.1pg/ml
  • Standard:54 pg/ml
  • Price:$498
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameRat Prothrombin Fragment 1+2 (F1+2) ELISA Kit

Purpose

Our Rat Prothrombin Fragment 1+2 (F1+2) ELISA kit is to assay F1+2 levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to F1+2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for F1+2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain F1+2 and HRP conjugated F1+2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of F1+2. You can calculate the concentration of F1+2 in the samples by comparing the OD of the samples to the standard curve.

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