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Human Defensin Beta 4 (DEFb4)ELISA Kit

Human Defensin Beta 4 (DEFb4)ELISA Kit

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  • Cat NO.:SL3162Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:6pg/ml-400 pg/ml
  • Sensitivity:1.2pg/ml
  • Standard:540pg/mL
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameHuman Defensin Beta 4 (DEFb4)ELISA Kit

Purpose

Our Human Defensin Beta4 (DEFb4)ELISA kit is to assay Defensin Beta 4 (DEFb4)  levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 4 (DEFb4). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for  Defensin Beta 4 (DEFb4) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Defensin Beta 4 (DEFb4) and HRP conjugated Defensin Beta 4 (DEFb4) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Defensin Beta 4 (DEFb4) ,You can calculate the concentration of Defensin Beta 4 (DEFb4) in the samples by comparing the OD of the samples to the standard curve.

 

 

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