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Mouse Neuron-specific enolase(NSE) ELISA Kit

Mouse Neuron-specific enolase(NSE) ELISA Kit

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  • Cat NO.:SL0860Mo
  • Product Spec:96T
  • Wavelength:450nm
  • Assay range:15pg/ml -1000 pg/ml
  • Sensitivity:3.3 pg/ml
  • Standard:1350 pg/ml
  • Sample Volume:50ul
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameMouse Neuron-specific enolase(NSE) ELISA Kit

 

Purpose

Our Mouse Neuron-specific enolase(NSE) ELISA kit is to assay NSE levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NSE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for NSE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NSE and HRP conjugated NSE antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NSE. You can calculate the concentration of NSE in the samples by comparing the OD of the samples to the standard curve.

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