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Human Excision Repair Cross-Complementation Group 4(ERCC4)ELISA Kit

Human Excision Repair Cross-Complementation Group 4(ERCC4)ELISA Kit

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  • Cat NO.:SL3416Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:30 pg/ml - 2000 pg/ml
  • Sensitivity:5 pg/ml
  • Standard:2700 pg/ml
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameHuman Excision Repair Cross-Complementation Group 4 (ERCC4) ELISA Kit

Purpose

Our Human Excision Repair Cross-Complementation Group 4 (ERCC4) ELISA kit is to assay ERCC2 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ERCC4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for ERCC4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ERCC4 and HRP conjugated ERCC4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ERCC4. You can calculate the concentration of ERCC4 in the samples by comparing the OD of the samples to the standard curve.

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