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Porcine Brucella Antibody IgG(Brucella-IgG)  ELISA Kit

Porcine Brucella Antibody IgG(Brucella-IgG) ELISA Kit

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  • Cat NO.:SL0255Po
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Qualitative:Negative/Positive
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NamePorcine Brucella Antibody IgG(Brucella-IgG) ELISA Kit

Purpose

Our Porcine Brucella Antibody IgG(Brucella-IgG)ELISA kit is for the qualitative 

determination of Brucella-IgG levels in Porcine serum, plasma, culture media or any

biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Brucella-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Brucella is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Brucella-IgG and HRP conjugated Brucella antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Brucella-IgG is determined by comparing with the CUTOFF value.

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