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Human Trefoil Factor 3 (TFF3) ELISA Kit

Human Trefoil Factor 3 (TFF3) ELISA Kit

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  • Cat NO.:SL3050Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:10pg/ml -800 pg/ml
  • Sensitivity:2.2pg/ml
  • Standard:1080 pg/ml
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameHuman Trefoil Factor 3 (TFF3)ELISA Kit

Purpose

Our Human Trefoil Factor 3 (TFF3)ELISA kit is to assay Trefoil Factor 3 (TFF3) levels in serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 3 (TFF3). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for  Trefoil Factor 3 (TFF3) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Trefoil Factor 3 (TFF3) and HRP conjugated Trefoil Factor 3 (TFF3)antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Trefoil Factor 3 (TFF3),You can calculate the concentration of Trefoil Factor 3 (TFF3) in the samples by comparing the OD of the samples to the standard curve.

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