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Bovine oxytocin ELISA Kit

Bovine oxytocin ELISA Kit

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  • Cat NO.:SL0165Bo
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:0.3pg/ml -20pg/ml
  • Sensitivity:0.05pg/ml
  • Standard:27 pg/ml
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Purpose

Our Bovine oxytocin(OT)ELISA kit is to assay oxytocin(OT) levels in Bovine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to oxytocin(OT)Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for oxytocin(OT)is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain oxytocin(OT)and HRP conjugated oxytocin(OT)antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of oxytocin(OT)You can calculate the concentration of oxytocin(OT) in the samples by comparing the OD of the samples to the standard curve.

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