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Porcine Erysipelothrix rhusiopathiae  antibody (E.rhusiopathiae- Ab) ELISA Kit

Porcine Erysipelothrix rhusiopathiae antibody (E.rhusiopathiae- Ab) ELISA Kit

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  • Cat NO.:SL0247Po
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Qualitative:Negative/Positive
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NamePorcine Erysipelothrix rhusiopathiae antibody (E.rhusiopathiae-Ab) 

ELISA Kit

Purpose

Our Porcine Erysipelothrix rhusiopathiae antibody (E.rhusiopathiae-Ab) ELISA kit is for the qualitative determination of E.rhusiopathiae-Ab levels in Porcine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to E.rhusiopathiae-Ab Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for E.rhusiopathiae-Ab is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain E.rhusiopathiae-Ab and HRP conjugated E.rhusiopathiae-Ab antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of E.rhusiopathiae-Ab is determined by comparing with the CUTOFF value.

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