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Human extractable nuclear antigen(ENA) ELISA Kit

Human extractable nuclear antigen(ENA) ELISA Kit

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  • Cat NO.:SL2798Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Qualitative:Qualitative
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Purpose

Our Human extractable nuclear antigen(ENA) ELISA kit is to assay extractable nuclear antigen(ENA) levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to  extractable nuclear antigen(ENA) . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for extractable nuclear antigen(ENA)  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain extractable nuclear antigen(ENA) and HRP conjugated  extractable nuclear antigen(ENA) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of extractable nuclear antigen(ENA) is determined by comparing with the CUTOFF value.

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