Aliases:	NADH:Ubiquinone Oxidoreductase Subunit B9; UQOR22; LYRM3; B22; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 9, 22kDa; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 9; NADH-Ubiquinone Oxidoreductase B22 Subunit; LYR Motif-Containing Protein 3; Complex I B22 Subunit; CI-B22; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 9 (22kD, B22); Complex I-B22; MC1DN24
Background:	UniProt Entry: Q9Y6M9;NCBI Gene Entry: 4715
Application Information	Molecular Weight: Predicted, 22 kDa, observed, 22 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB90 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NDUFB9
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NDUFB9 expression in C2C12 cells using NDUFB9 antibody 1:2,000. Green, isotype control; red, NDUFB9.

Immunocytochemical staining of C2C12 cells with anti-NDUFB9 antibody 1:1,000. Nuclei were stained blue with DAPI; NDUFB9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-NDUFB9 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NDUFB9 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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