Aliases:	NDUFS3; NADH:Ubiquinone Oxidoreductase Core Subunit S3; NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 3, Mitochondrial; CI-30; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3, 30kDa (NADH-Coenzyme Q Reductase); NADH-Ubiquinone Oxidoreductase 30 KDa Subunit; Complex I 30kDa Subunit; Complex I-30kD; CI-30kD; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3 (30kD) (NADH-Coenzyme Q Reductase); NADH Dehydrogenase-Ubiquinone 30 KDa Subunit; EC 1.6.99.5; EC 7.1.1.2; MC1DN8
Background:	UniProt Entry: O75489;NCBI Gene Entry: 4722
Application Information	Molecular Weight: Predicted, 30 kDa, observed, 26 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2145 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NDUFS3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NDUFS3 expression in HepG2 cells using NDUFS3 antibody 1:2,000. Green, isotype control; red, NDUFS3.

Immunocytochemical staining of HepG2 cells with NDUFS3 antibody 1:1,000. Nuclei were stained blue with DAPI; NDUFS3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NDUFS3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NDUFS3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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