Aliases:	NIMA Related Kinase 6; SID6-1512; NIMA (Never In Mitosis Gene A)-Related Kinase 6; Putative Serine-Threonine Protein Kinase; Serine/Threonine-Protein Kinase Nek6; Never In Mitosis A-Related Kinase 6; NimA-Related Protein Kinase 6; Protein Kinase SID6-1512; EC 2.7.11.34; EC 2.7.11.1
Background:	UniProt Entry: Q9HC98;NCBI Gene Entry: 10783
Application Information	Molecular Weight: Predicted, 36 kDa, observed, 28 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB755 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NEK6
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NEK6 expression in HT-1080 cells using NEK6 antibody1:2,000. Green, isotype control; red, NEK6.

Immunocytochemical staining of HT-1080 cells with anti-NEK6 antibody 1:1,000. Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NEK6 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NEK6 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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