Purpose

Our Canine Gamma-Glutamyltransferase 7(GGT7) ELISA kit is to assay

Gamma-Glutamyltransferase 7 (GGT7) levels in Canine serum, plasma, culture media or any

biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided

in this kit has been pre-coated with an antibody specific to Gamma-Glutamyltransferase 7

(GGT7) . Standards or samples are added to the appropriate Microelisa stripplate wells and

combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated

antibody specific for Gamma-Glutamyltransferase 7 (GGT7) is added to each Microelisa

stripplate well and incubated. Free components are washed away. The TMB substrate solution

is added to each well. Only those wells that contain Gamma-Glutamyltransferase 7 (GGT7) and

HRP conjugated Gamma-Glutamyltransferase 7 (GGT7) antibody will appear blue in color and

then turn yellow after the addition of the stop solution. The optical density (OD) is measured

spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the

concentration of Gamma-Glutamyltransferase 7 (GGT7) . You can calculate the concentration

of Gamma-Glutamyltransferase 7 (GGT7) in the samples by comparing the OD of the samples

to the standard curve.

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