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Drug Names
Generic Name:Poultry Fowl Cholera Antibody ELISA Kit
Purpose
Our Poultry Fowl Cholera Antibody ELISA kit is to assay Fowl Cholera levels in Poultryserum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Fowl Cholera. Standards or samples are added to the Fowl Choler aopriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Fowl Cholera is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Fowl Cholera and HRP conjugated Fowl Cholera ntigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Fowl Cholera. You can calculate the concentration of Fowl Cholera in the samples by comparing the OD of the samples to the standard curve.
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