Intended use
This immunoassay kit allows for the in vitro quantitative determination of General BHA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General BHA, During the reaction, General BHA in the sample or standard competes with a fixed amount of biotin-labeled General BHA for sites on a pre-coated Monoclonal antibody specific to General BHA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General BHA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta-Hydroxybutyric acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-111%	114-126%	82-92%	92-101%
EDTA plasma(n=5)	99-110%	102-112%	102-112%	90-100%
heparin plasma(n=5)	94-103%	99-108%	101-111%	103-113%

 

General Annotation

 

Sub Unit:

N/A

 

Function:

In humans, beta-hydroxybutyrate is synthesized in the liver from acetyl-CoA in a reaction catalyzed by the enzyme beta-hydroxybutyrate dehydrogenase and can be used as an energy source by the brain when blood glucose is low. Diabetic patients can have their ketone levels tested via urine or blood to indicate diabetic ketoacidosis.

 

Location:

beta-Hydroxybutyric acid (also known as beta-hydroxybutyrate or 3-hydroxybutyric acid) is a ketone body. It is a chiral compound having two enantiomers, D-3-hydroxybutyric acid and L-3-hydroxybutyric acid. Like the other ketone bodies (acetoacetate and acetone), levels of beta-hydroxybutyrate are raised in ketosis.

 

 

Sequence:C4H8O3

 

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