Drug Names

Generic Name：Chicken Inclusion Body Hepatitis (IBH)ELISA Kit

Purpose

This kit allows for the determination of IBH concentrations in Chicken serum, blood plasma, and other biological fluids.

Principle

The kit assay Chicken IBH level in the sample，use Purified Chicken IBH antibody to coat microtiter plate wells, make solid-phase antibody, then add IBH to wells, Combined With IBH, after washing and removing non-combinative antigen and other components ,then Combined IBH antibody which with HRP labeled become antibody - antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge IBH exist in the sample or not.

 

 

 

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