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Purpose
Our Chicken Zonula occludens-1 (ZO1) ELISA kit is to assay Zonula occludens-1 (ZO1) levels in Chicken serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Zonula occludens-1 (ZO1) Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Zonula occludens-1 (ZO1) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Zonula occludens-1 (ZO1) and HRP conjugated Zonula occludens-1 (ZO1)antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Zonula occludens-1 (ZO1). You can calculate the concentration of Zonula occludens-1 (ZO1) in the samples by comparing the OD of the samples to the standard curve.
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