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Determination principle:Glycolic acid oxidase catalytic glycolic acid oxidation to form acetal acid, acetical acid and phenyl hydrochloride reaction to form acetaganogenate,324 nm has a feature absorption peak.
Instruments, consumables:
Balance, low temperature centrifuge, ultraviolet lightometer / enzyme symbol, trace quantum-based cuvette / 96-well plate.
Measuring significance:
Glycolic oxidase (EC1.1.3.1) is a critical enzyme in plant light breathing metabolism, also a key enzyme of synthetic oxalic acid, which catalyzes glycolic oxidationGenerate acetical acid, the study of the photoreceptic metabolism and its regulation of significant meanings: glycolic acid oxidase catalyzed glyoxylate, glyoxylic acid and phenyl hydrochloride reaction to form acetal acid benzene, at 324 nmThere is a feature absorption peak.Need instruments, consumables: balance, low temperature centrifuge, ultraviolet spectrophotometer / enzyme label, trace quantum-based cuvette / 96-well plate.
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