Summary:

 

Purpose

 

Our Quick Step Human membrane Immunoglobulin D (MIgD)elisa kit is to assay MIgD  levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to MIgD . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for MIgD  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MIgD  and HRP conjugated MIgD  antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MIgD . You can calculate the concentration of MIgD  in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2714Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μg/ml	0.043	0.0460
		0.049
S1	9μg/ml	1.933	1.9385
		1.944
S2	6μg/ml	1.394	1.4015
		1.409
S3	3μg/ml	0.713	0.7095
		0.706
S4	1.5μg/ml	0.400	0.3970
		0.394
S5	0.75μg/ml	0.206	0.2125
		0.219

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