Summary:

 

Purpose

 

Our Quick Step Human 21-Hydroxylase (21-OH) Autoantibody ELISA Kit is to assay 21-OH Autoantibody levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to 21-OH Autoantibody. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for 21-OH Autoantibody is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 21-OH Autoantibody and HRP conjugated 21-OH Autoantibody antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 21-OH Autoantibody. You can calculate the concentration of 21-OH Autoantibody in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3645Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pmol/L	0.063	0.0580
		0.053
S1	12pmol/L	1.955	1.9585
		1.962
S2	8pmol/L	1.288	1.2920
		1.296
S3	4pmol/L	0.704	0.7080
		0.712
S4	2pmol/L	0.387	0.3965
		0.406
S5	1pmol/L	0.196	0.2000
		0.204

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