Summary:

 

Purpose

 

Our Quick Step Rat Humanin ELISA Kit is to assay Humanin levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Humanin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Humanin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Humanin and HRP conjugated Humanin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Humanin. You can calculate the concentration of Humanin in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1800Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0单位	0.044	0.0480
		0.052
S1	150单位	1.967	1.9630
		1.959
S2	100单位	1.328	1.3195
		1.311
S3	50单位	0.692	0.7030
		0.714
S4	25单位	0.392	0.3875
		0.383
S5	12.5单位	0.197	0.1900
		0.183

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