Summary:

 

Purpose

 

Our Quick Step Rabbit Immunoglobulin M,IgM ELISA Kit is to assay IgM levels in Rabbit serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IgM and HRP conjugated IgM antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IgM. You can calculate the concentration of IgM in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0000Rb is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Blank Well	0pg/ml	0.049	0.0440
		0.039
S1	1800pg/ml	1.956	1.9530
		1.950
S2	1200pg/ml	1.389	1.3910
		1.393
S3	600pg/ml	0.709	0.7135
		0.718
S4	300pg/ml	0.390	0.3875
		0.385
S5	150pg/ml	0.195	0.2000
		0.205

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