SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
SENSITIVITY	44.07 pg/mL
RANGE	125 pg/mL - 8000 pg/mL
ASSAY TIME	1.5 h
RECOVERY	78 % - 107 %
AVERAGE RECOVERY	0.99
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL10 monoclonal antibody Mouse IL10 freeze-dried standard IL10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL10 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL10 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL10 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL10 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

 

 

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