SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
SENSITIVITY	0.35 pg/mL
RANGE	3.125 pg/mL – 200 pg/mL
ASSAY TIME	1.5 h
RECOVERY	88% – 114%
AVERAGE RECOVERY	0.99
INTRA PRECISION	3.4% – 4.6%
INTER-PRECISION	3.1% – 5.4%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL18 monoclonal antibody Human IL18 freeze-dried standard IL18 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

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