SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 10μL; DMEM cell culture supernatant: 100μL
SENSITIVITY	11.71 pg/mL
RANGE	15.63 pg/mL – 1000 pg/mL
ASSAY TIME	1.5 h
RECOVERY	90% – 107%
AVERAGE RECOVERY	0.91
INTRA PRECISION	3.9% – 5.7%
INTER-PRECISION	4.5% – 7.3%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL10 monoclonal antibody Human IL10 freeze-dried standard IL10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human IL-10 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-10 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-10 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-10 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader

 

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Totalgoods,subtotals:￥Checkout