SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 10μL; DMEM cell culture supernatant: 33μL
SENSITIVITY	1.77 pg/mL
RANGE	3.13 pg/mL – 200 pg/mL
ASSAY TIME	1.5 h
RECOVERY	100% – 122%
AVERAGE RECOVERY	1.08
INTRA PRECISION	4.2% – 5.4%
INTER-PRECISION	5.5% – 6.2%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody Human TNF-α freeze-dried standard TNF-α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human TNF-α has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for TNF-α (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then TNF-α present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of TNF-α bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader.

 

Cartpieces

• 
• 1

  1Delete

• 
• 6

  6Delete

• 
• O

  ODelete

• 
• O

  ODelete

• 
• +

  +Delete

• 
• 2

  2Delete

• 
• 2

  2Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 6

  6Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• D

  DDelete

• 
• D

  DDelete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• 
• i

  iDelete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• d

  dDelete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 6

  6Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• 
• w

  wDelete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• O

  ODelete

• 
• 5

  5Delete

• 
• S

  SDelete

Totalgoods,subtotals:￥Checkout