Cat NO.:	HS-EL0261Hu
Product:	Sunlong Medical™ Human IL-10 High Sensitivity ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	88% - 100%
Mean Spike Recovery:	0.92
CV of Intra plate:	3.5% - 4.1%
CV of Inter plate:	4.0% - 8.8%
NCBI_Gene:	3586
UniProtKB:	P22301

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody Human IL-10 freeze-dried standard IL-10 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-10 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-10 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

1.The Effect of an Extract of Sappanwood, Protosappanin A and Protosappanin B on Osteogenesis in Periodontitis

 

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3.CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages

 

4.Single-cell transcriptomics reveals variations in monocytes and Tregs between gout flare and remission

 

5.α5-nAChR/STAT3/CD47 axis contributed to nicotine-related lung adenocarcinoma progression and immune escape

 

6.Orosomucoid 1 promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing macrophage M2 polarization

 

7.Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro

 

8.Parthenolide alleviates microglia‐mediated neuroinflammation via MAPK/TRIM31/NLRP3 signaling to ameliorate cognitive disorder

 

9.The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study

 

10.A role of NR4A2 in Graves’ disease: regulation of Th17/Treg

 

11.BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study

 

12.hPMSCs Regulate the Level of TNF-α and IL-10 in Th1 Cells and Improve Hepatic Injury in a GVHD Mouse Model via CD73/ADO/Fyn/Nrf2 Axis

 

13.Potentiated effects of lactate receptor GPR81 on immune microenvironment in breast cancer

 

14.MicroRNA-143-3p ameliorates collagen-induced arthritis by polarizing naive CD4+T cells into Treg cells

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