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Sunlong Medical™ Mouse Endoglin/CD105 ELISA Kit
Sunlong Medical™ Mouse Endoglin/CD105 ELISA Kit
ENG elisa kit | endoglin elisa kit | AI528660 | AI662476 | CD105 | Endo | expressed sequence AI528660 | expressed sequence AI662476
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  • NO.:EL0085Mo
    Species:Mouse
    Assay range:0.16ng/mL-10ng/mL
    Sensitivity:10.58 pg/mL
    Sample Volume:20 μL
    Assay type:Sandwich Method
    Validity:Two Years
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Details

Cat NO.:
EL0085Mo
Product:
Sunlong Medical™ Mouse Endoglin/CD105 ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
81% - 107%
Mean Spike Recovery:
0.99
CV of Intra plate:
2.0 % - 5.3 %
CV of Inter plate:
4.1 % - 6.0 %
NCBI_Gene:
13805
UniProtKB:
Q63961
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Endoglin/CD105 monoclonal antibody
Mouse Endoglin/CD105 freeze-dried standard
Endoglin/CD105 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Endoglin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, Endoglin present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Endoglin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 

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