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Sunlong Medical™ Human Cystatin C ELISA Kit
Sunlong Medical™ Human Cystatin C ELISA Kit
CST3 elisa kit | cystatin C elisa kit | ARMD11 | bA218C14.4 cystatin C | cystatin 3 | cystatin C amyloid angiopathy and cerebral hemorrhage | cystatin-3 | cystatin-C | epididymis secretory protein Li 2 | gamma-trace | HEL-S-2 | MGC117328 | neuroendocr
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  • NO.:EL0254Hu
    Species:Human
    Assay range:31.25pg/mL-2000pg/mL
    Sensitivity:10.57 pg/mL
    Sample Volume:100 μL
    Assay type:Sandwich Method
    Validity:Two Years
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Citations

Cat NO.:
EL0254Hu
Product:
Sunlong Medical™ Human Cystatin C ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cellular supernatant
Spike Recovery Range:
85% - 115%
Mean Sike Recovery:
0.98
CV of Intra plate:
3.3 % - 7.9 %
CV of Inter plate:
3.1 % - 6.2 %
NCBI_Gene:
1471
UniProtKB:
P01034

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Cystatin C monoclonal antibody
Human Cystatin C freeze-dried standard
Cystatin C detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Cystatin C antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Cystatin C present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Cystatin C in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 1. Microcystin-LR-Induced Interaction between M2 Tumor-Associated Macrophage and Colorectal Cancer Cell Promotes Colorectal Cancer Cell Migration through Regulating the Expression of TGF-β1 and CST3

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2023-06-23
 
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH 2023-09-22

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